This is the Evergreen CBSE Biology Lab Manual for Class 12, prepared for the practical component of the CBSE Class XII biology course. It is an English medium (Roman script) book — the complete text, procedures, observations, diagrams and viva questions are in English, and there is no Hindi content in it. The cover states that it follows the syllabus issued by CBSE on 21.04.2022 vide Circular No. Acad-48/2022, and web support is available. The biology practical carries 30 marks in Class 12 and is examined differently from physics or chemistry. Alongside the experiments there is a spotting section — the student is shown prepared slides and specimens and asked to identify them and give the identifying features on the spot. That section is where marks are most often lost, and it cannot be prepared from a theory textbook. This manual sets out each prescribed experiment in the form the examiner expects in the record file: aim, requirements, principle, procedure, observation, result and precautions. The experiments include study of pollen germination, effect of different factors on the rate of transpiration, study of soil and water samples for pH and turbidity, isolation of DNA from plant material, and the study of prepared slides of mitosis and meiosis. The spotting section covers the specimens and slides prescribed for identification — flowers adapted to different pollination modes, pollen germination, T.S. of blastula, mendelian inheritance, common disease-causing organisms, and models of homologous and analogous organs — with labelled diagrams and the identifying points to be written. The viva voce section, arranged experiment by experiment, covers what the external examiner actually asks, and the diagram work throughout is what a student copies into the practical file. The manual is used alongside the NCERT Class 12 biology textbook, which carries the theory the practicals rest on. Key Features: English medium book — complete content written in English (Roman script), no Hindi content Published by Evergreen Publications CBSE Biology Lab Manual for Class 12 (Class XII) Strictly according to the new syllabus issued by CBSE on 21.04.2022 vide Circular No. Acad-48/2022 Covers all prescribed Class 12 biology experiments Each experiment set out with aim, requirements, principle, procedure, observation, result and precautions Covers study of pollen germination on a slide Covers the effect of different factors on the rate of transpiration Covers study of soil and water samples — pH, turbidity and analysis Covers isolation of DNA from plant material Covers study of prepared slides of mitosis and meiosis Spotting section with labelled diagrams and identifying features Covers slides and specimens prescribed for identification Viva voce questions with answers, arranged experiment by experiment Diagrams in the form required for the practical record file Project work guidance included Web support available For the 30-mark Class 12 biology practical examination Used alongside the NCERT Class 12 biology textbook यह पुस्तक एवरग्रीन पब्लिकेशन्स द्वारा CBSE कक्षा 12 जीव विज्ञान के प्रायोगिक पाठ्यक्रम हेतु तैयार की गई प्रयोगशाला पुस्तिका (Biology Lab Manual) है। ध्यान दें: यह पुस्तक पूर्णतः अंग्रेज़ी भाषा एवं रोमन लिपि में है — इसमें हिन्दी भाषा का प्रयोग नहीं है। यह CBSE द्वारा 21.04.2022 को परिपत्र संख्या Acad-48/2022 के अंतर्गत जारी नवीन पाठ्यक्रम के अनुसार है। इसमें प्रत्येक निर्धारित प्रयोग उसी प्रारूप में दिया गया है जो परीक्षक रिकॉर्ड फाइल में अपेक्षित करता है — उद्देश्य, आवश्यक सामग्री, सिद्धांत, विधि, प्रेक्षण, परिणाम एवं सावधानियाँ। परागकण अंकुरण, वाष्पोत्सर्जन दर को प्रभावित करने वाले कारक, मृदा एवं जल के नमूनों का परीक्षण (pH एवं मलिनता), पादप सामग्री से DNA पृथक्करण तथा समसूत्री एवं अर्धसूत्री विभाजन की स्थायी स्लाइडों का अध्ययन सम्मिलित हैं। कक्षा 12 जीव विज्ञान की प्रायोगिक परीक्षा 30 अंकों की होती है और इसमें स्पॉटिंग (Spotting) खंड सर्वाधिक अंक-निर्धारक होता है — इस पुस्तक में स्पॉटिंग हेतु निर्धारित स्लाइडों एवं नमूनों के नामांकित चित्र तथा पहचान-बिंदु दिए गए हैं। प्रत्येक प्रयोग के साथ मौखिक परीक्षा (viva voce) प्रश्न-उत्तर भी सम्मिलित हैं।